Preparation of amino acid mixtures for cell-free expression systems
نویسندگان
چکیده
منابع مشابه
Preparation of amino acid mixtures for cell-free expression systems.
Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all Escherichia coli cell-free expression system. Our method is fast, easy to execute, and economically advantageous compared to expens...
متن کاملCell-free expression with the toxic amino acid canavanine.
Canavanine is a naturally occurring noncanonical amino acid, which is analogous to arginine. It is a potent antimetabolite and natural allelochemic agent, capable of affecting or blocking regulatory and catalytic reactions that involve arginine. Incorporated into proteins at arginine positions, canavanine can be detrimental to protein stability and functional integrity. Although incorporation o...
متن کاملEnantioenrichment in sublimed amino acid mixtures.
A real amplification of an initial enantiomeric excess can be detected when two amino acids are sublimed at high temperature, even if one of the components is a racemic compound that does not convert into a conglomerate by sublimation.
متن کاملPreparation of photoactivable amino acid derivatives.
A range of N-protected-alpha-amino acyl-5,7-dinitroindolines 3a-z were prepared in good yields from commercially available N-protected-alpha-amino acids 1a-z by a two-step sequence of acylation and intramolecular amide N-arylation. Subsequent photochemical acylation of the N-protected-alpha-amino acyl-5,7-dinitroindolines 3e,g,r afforded the corresponding N-protected-alpha-amino acid amides 22e...
متن کاملDifferences in Chloramphenicol Sensitivity of Cell-free Amino Acid Polymerization Systems.
In contrast to other inhibitors, particularly puromycin, chloramphenicol has a rather variable effect on cell-free systems for protein synthesis of different species. At concentration levels of 1O-5 to low4 M, which are comparable to levels in vivo, bacterial systems are inhibited 75 to 90% whereas those from animal tissue (1, 2) or yeast (3) seem not to be affected. It is reported (4) that pro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2015
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114249